Detection and discrimination of latent and replicative herpesvirus infection at the single cell level in vivo.

نویسندگان

  • L L Decker
  • G J Babcock
  • D A Thorley-Lawson
چکیده

1 Epstein-Barr Virus: The B95-8 Strain Map Paul J. Farrell ......................................................................................... 3 2 Analysis of Replication of oriP-Based Plasmids by Quantitative, Competitive PCR Ann L. Kirchmaier ................................................................................ 13 3 Genetic Analysis and Gene Expression with Mini-Epstein-Barr Virus Plasmids Ellen Kilger and Wolfgang Hammerschmidt .................................... 23 4 Construction of cDNA Libraries for the Analysis of the Structure of Complementary Strand Transcripts (CSTs) Paul R. Smith........................................................................................ 37 5 Analysis of the Expression and Function of the EBV-Encoded Small RNAs, the EBERs, in Heterologous Cells Kenneth G. Laing, Volker Matys, and Michael J. Clemens ............ 45 6 Visualizing EBV Expression Patterns by FISH Anna Szeles .......................................................................................... 67

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Isolation of Bovine Herpesvirus-1 (BoHV-1) From Latently Infected/Carrier Cattle in Ahvaz

Bovine herpesvirus 1 (BoHV-1) is one of the most important infectious viruses of cattle which is considered as the causative agent of several clinical conditions in this species. Hence, we tried to isolate a local strain of the virus from carrier cattle in Ahvaz based on a strategy for reactivation of the latent infection in hosts having antibodies against the virus. Thus, 20 cattle were examin...

متن کامل

Detection and Discrimination of Theileria annulata and Theileria lestoquardi by using a Single PCR

  The aim of this study was to detect and differentiate Theileria annulata and T. lestoquardi (hirci) by PCR. Members of the genus Theileria are tick-borne hemoprotozoan parasites those cause fatal and enervating diseases of cattle and sheep in Iran . In order to develop a specific method for detecting and identification of Theileria species, specific primers from the surface protein (SP) seque...

متن کامل

Detection of infectious laryngotracheitis virus (Gallid herpesvirus-1) from clinically infected chickens in Egypt by different diagnostic methods

Infectious laryngotracheitis (ILT) disease is an acute highly contagious viral disease leading to massive economic losses to the national poultry industry. This study aimed to identify the most accurate and rapid diagnostic methods to rescue layer poultry farms from intense outbreaks in Egypt. Fifty pathological specimens were collected and subjected to virus isolation (VI), histopathology, dir...

متن کامل

PCR detection of thymidine kinase gen of latent herpes simplex Virus type 1 in mice trigeminal ganglia

  Herpes simplex virus type 1 establishes a latent infection in the peripheral nervous system following primary infection. During latent infection, virus genome exhibit limited transcription, with the HSV LATs consistently detected in latency infected ganaglia. Following ocular infection viral latency develops in the trigeminal ganglia. In this study PCR has been used for detection of HSV-1 nuc...

متن کامل

DETECTION AND RESTRICTION ANALYSIS OF C YTOMEGALOVIRUS DNA PERSISTING IN HUMAN ATHEROSCLEROTIC PLAQUES USING POLYMERASE CHAIN REACTION

The polymerase chain reaction (PCR) as applied to detection of a foreign DNA in clinical specimens could provide a sensitive instrument for rapid detection of viral DNA persisting in tissues of patients suspected of latent infection. Human cytomegalovirus (HCMV) DNA was found in arterial plaques of patients with atherosclerotic lesions using a PCR assay with nested primer oligonucleotides ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Methods in molecular biology

دوره 174  شماره 

صفحات  -

تاریخ انتشار 2001